Resources

Links

CRISPR/Cas9 in Drosophila:

FlyBase: CRISPR

flyCRISPR discussion group

CRISPRflydesign website

OXfCRISPR website

FlyCas9 website

Drosophila CRISPR target finders:

flyCRISPR Optimal Target Finder

DRSC CRISPR finder

E-CRISP

 

General CRISPR/Cas9 resources:

CRISPR discussion group

Zhang lab CRISPR website

Joung lab CRISPR website

Goldstein lab CRISPR website

 

Publications

Bier, E., Harrison, M.M., O'Connor-Giles, K.M. and Wildonger, J. Advances in Engineering the Fly Genome with the CRISPR-Cas System. FlyBook. GENETICS. In press.

Akbari, B. O., Bellen, H. J., Bier, E., Bullock, S. L., Burt, A., Church, G. M., Cook, K. R., Duchek, P., Edwards, O. R., Esvelt, K. M., Gantz, V. M., Golic, K. G., Gratz, S. J., Harrison, M. M., Hayes, K. R., James, A. A., Kaufman, T. C., Knoblich, J., Malik, H. S., Matthews, K. A., O'Connor-Giles, K. M., Parks, A. L., Perrimon, N., Port, R., Russell, S., Ueda, R., and Wildonger, J. (2015). Safeguarding gene drive experiments in the laboratory. Science 349, 927-929.

Gratz, S.J., Rubinstein, C.D., Harrison, M.M., Wildonger, J., and O'Connor-Giles, K.M. (2015). CRISPR-Cas9 Genome Editing in Drosophila. Current Protocols in Molecular Biology 111, 31 32 31-31 32 20.

Gratz, S.J., Harrison, M.M., Wildonger, J., and O'Connor-Giles, K.M. (2015). Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9. Methods in Molecular Biology 1311, 335-348.

Harrison, M.M., Jenkins, B.V., O'Connor-Giles, K.M., and Wildonger, J. (2014). A CRISPR view of development. Genes & Development 28, 1859-1872.

Gratz, S.J.*, Ukken, F.P.*, Rubinstein, C.D., Thiede, G., Donohue, L.K., Cummings, A.M., and O'Connor-Giles, K.M. (2014). Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila. Genetics 196, 961-971.*These authors contributed equally.

Gratz, S.J., Wildonger, J., Harrison, M.M., and O'Connor-Giles, K.M. (2013b). CRISPR/Cas9-mediated genome engineering and the promise of designer flies on demand. Fly 7, 249-255.

Gratz, S.J., Cummings, A.M., Nguyen, J.N., Hamm, D.C., Donohue, L.K., Harrison, M.M., Wildonger, J., and O'Connor-Giles, K.M. (2013). Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease. Genetics 194(4), 1029-1035.